Serologic proteome analysis of human lung adenocarcinoma
- VernacularTitle:人肺腺癌组织的血清蛋白质组学研究
- Author:
Cui LI
;
Zhiqiang XIAO
;
Chaojun DUAN
;
Cane TANG
;
Hong YI
;
Zhuchu CHEN
- Publication Type:Journal Article
- Keywords:
Lung neoplasms/ pathology;
Adenocarcinoma/ pathology;
Proteomics;
Electrophoresis,gel,two-dimentional;
Spectrometry,mass matrix-assisted laser desorption-ionization
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify lung adenocarcinoma-associated antigens by using Serologic Proteome Analysis(SERPA),an approach which combined conventional proteome analysis with serological screening.Methods SERPA of four human lung adenocarcinoma tissues were performed.The Western blot imaging films which reacted with autologous patient serum and with control normal serum were obtained and the differential reacting protein spots were recognized.Results Well-resolved,reproducible 2-DE Western blot imaging films of human lung adenocarcinoma reacted with autologous patient sera and the control sera were obtained.Totally(27?5) differentially expressed proteins which only were reactive with lung adenocarcinoma patient sera were found.Some differentially expressed proteins were identified by peptide mass fingerprint(PMF).Some of the proteins were the products of oncogenes,and others were involved in the regulation of cell cycle and signal transduction.Conclusion These results will provide scientific foundation on screening the molecular biomarker for the diagnosis,treatment,and prognosis of the lung adenocarcinoma.
