Morphological study of human mesenchymal stem cells after exposure to extracorporeal shock wave
- VernacularTitle:体外冲击波干预成人骨髓间充质干细胞的形态学研究
- Author:
Wuzhou WANG
;
Gengyan XING
;
Ming JIANG
;
Xiaodong BAI
;
Chuan JIANG
- Publication Type:Journal Article
- Keywords:
High-energy shock waves;
Mesenchymal stem cells
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the morphological changes of human mesenchymal stem cells(hMSCs) after exposure to extracorporeal shock wave(ESW).Methods An optimal experimental energy dose of ESW was applied to hMSCs of passage one.The morphological changes were examined by using inverted phase contrast microscope(IPCM),transmission electron microscope and cytochemical staining.Results ESW of 5kV and 100 times could raise the viability and proliferation of the cells.In the hMSCs of passage 3-5,the multiplicative division was speeded up,and the cells in the nuclear fission phase were increased.In the hMSCs of passage 7-9,the volume of the cells increased,and the endoplasmic reticulum was expanded.The Golgi's apparatus was developed and the matrix of the cells was bunchy.In the hMSCs of passage 11,the multiplicative division was also detected.Conclusion The proliferation and differentiation of the hMSCs can be improved by the treatment of ESW.
