Determination of Etoposide in Children with Leukemia by HPLC
- VernacularTitle:高效液相色谱法测定儿童白血病患者中依托泊苷血药浓度
- Author:
Xiaotong LU
;
Yuenian TANG
;
Jian ZHANG
;
Lixia LI
- Publication Type:Journal Article
- Keywords:
Etoposide;
Leukemia of children;
HPLC
- From:
China Pharmacy
2005;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish HPLC method for the determination of plasma etoposide level in children with leukemia. METHODS: The determination was performed on column Hypersil ODS. The mobile phase was water-methanol (45∶55) and the wavelength for detection was 284nm. RESULTS: The intraday recovery ranged from 93.56 to 96.24% with SD ranged at 1.07%～2.63%, and the inter-day recovery ranged from 92.85% to 94.26% with SD ranged at 3.55%～5.89%. CONCLUSION: This method is simple and sensitive, showed a good specificity, and suitable for the determination of etoposide in clinic samples.
