Evaluation of two methods in detection of platelet-associated autoantibody for the diagnosis of idiopathic thrombocytopenic purpura
- VernacularTitle:原发性血小板减少性紫癜两种血小板相关抗体检测方法的比较
- Author:
Linlin ZHANG
;
Yongqian JIA
;
Wenfang HUANG
- Publication Type:Journal Article
- Keywords:
Purpura,thrombocytopenic,idiopathic;
Flow cytometry;
Enzyme-linked immunosorbent assay;
Blood platelets;
Antibodies
- From:
International Journal of Laboratory Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the methods of measuring platelet-associated antibody PAIgG/ PAIgA/ PAIgM by flow cytometry(FCM) and enzyme linked immunosorbent assay(ELISA), and to investigate their diagnostic value for patients with idiopathic thrombocytopenic purpura(ITP).Methods With FCM and ELISA respectively, PAIg on the platelet membrane and in plasma were measured in 19 patients with ITP and 17 healthy volunteers, and were compared with each other in order to find out whether there were differences in these groups.Results FCM and ELISA measurement in patients with ITP were significantly higher than those in control group (P0.05). Compared with the results of ELISA, the positive percentage of PAIgG measured by FCM(84%) in ITP patients was slightly higher than that by ELISA(79%).Conclusion The platelet-associated antibodies of PAIgG/ PAIgA/ PAIgM, especially PAIgG,are important for diagnosing ITP. FCM, in combination with ELISA, may improve the reliability and the positive percentage of detection in ITP patients.
