Clone of S gene in occult hepatitis B virus infection and construction of prokaryotic expression vector
- VernacularTitle:隐匿性乙型肝炎病毒S基因的克隆及其表达载体的构建
- Author:
Fan WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Genes,viral;
Cloning,molecular;
Genetic rectors
- From:
International Journal of Laboratory Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the clone vector of S gene in occult hepatitis B virus infection.To analyse its mutations and to construct its prokaryotic expression vector.Methods To amplify the HBV S gene with polymerase chain reaction(PCR),recombinate the S gene and vector PUC18,sub-clone the recombination and construct its prokaryotic expression vector with pThiohis B. Results There were two samples negative with positive HBV DNA from 64 samples with negative hepatitis B surface antigen.The clone vector and prokaryotic fusion protein expression vector of an occult HBV infection′s S gene were successfully constructed.The S gene was not changed by sequencing analysis.Conclusion The fluorescence quantitive polymerase chain reaction (FQ-PCR) can examine low-copies in occult hepatitis B virus infection.Determining quantitivly HBV DNA of recombination can identify the recombination and display multiplication state intuitirely.It is very successful in constructing the clone vector and prokaryotic fusion protein expression vector.
