Detection of ascarid ITS-2 sequence out of intrahepatic bile duct stones by PCR technique
- VernacularTitle:应用PCR技术检测肝内胆管结石中蛔虫ITS-2序列
- Author:
Xigang CHEN
;
Wenpeng HUANG
;
Minhao PENG
;
Tao PENG
;
Dehong HU
;
Lequn LI
;
Dinghua YANG
- Publication Type:Journal Article
- Keywords:
Cholelithiasis;
Ascaris lumbricoides;
Molecular biology;
Polymerase chain reaction
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To search for the molecular figngerprint of ascaris lumbricoides in intrahepatic stones. Methods In 56 cases deoxyribonucleic acid (DNA) was exstracted from intrahepatic stones and surgically obtained bile duct tissues, stool and sercum examinations were performed. Based on the sequence and designed primers of ITS-2 of ascaris lumbricoides, polymerase chain reaction ( PCR) was performed. Results There were 12 positive results in 56 (21.4% ) cases of intrahepatic stones. Positive reaction appeared on the examinations of the stool on ascaris lumbricoides and the blood serum among 12 cases. Conclusions Ascaris lumbricoides DNA was found in human of intrahepatic stones, indicating that ascaris lumbricoides infection may play a role in the formation of intrahepatic stones.
