Study of hepatic cellular injury due to hepatic ischemia in dogs
- VernacularTitle:肝动脉缺血对犬肝细胞损害的实验研究
- Author:
Jiling JIANG
;
Wenjun YANG
;
Guozuo XIONG
;
Zhijie XU
;
Kui HE
;
Desong JIANG
;
Yong FANG
- Publication Type:Journal Article
- Keywords:
Liver/blood supply;
Ischemia;
Liver Artery;
Hepatic Cell Injury
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ischemic injury of hepatic cell caused by hepatic artery occlusion.Methods The hepatic artery was occluded in 20 dogs via operation,while the portal vein remained patent.Specimens were gained from the right liver at four time points:before occlusion of the hepatic artery,20(minutes),40 minutes and 60 minutes after artery occlusion.Each specimen was examined by HE and BCL-2 by immunohistochemistry.The gray scale of BCL-2 in HE sections was detected.Results Hepatic cellular injury was obvious 20 minutes after occlusion of the hepatic artery.Irreversible hepatic cellular injury was(observed) 60 minutes after hepatic artery occlusion.The results showed that the gray scale of BCL-2 at every time point after hepatic artery occlusion were significantly different from that before hepatic artery occlusion(P
