Effects of electrical stimulation of vagus nerve on gut injury following intestinal ischemia-reperfusion in rats
- VernacularTitle:迷走神经电刺激对大鼠肠缺血再灌注后肠损伤的影响
- Author:
Chuiliang LIU
;
Yujuan LI
;
Xiangyu LI
;
Kexuan LIU
;
Wenqi HUANG
- Publication Type:Journal Article
- Keywords:
Vagus nerve;
Intestines;
Reperfusion injury
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of electrical stimulation of vagus nerve on gut injury following intestinal ischemia-reperfusion in rats. METHODS: 30 adult male Wistar rats subjected to bilateral cervical vagotomy were randomly divided into three groups (n=10 per group): (1) Intestinal ischemia-reperfusion group (group I/R): laparotomy and I/R induced by clamping arteria mesenterica superior for 1 h followed by reperfusion for 2 h. (2) Vagus nerve stimulation group (group VNS): laparotomy, I/R and electric stimulation with pulse train of constant amplitude 5V, pulse width 2 ms and frequency 1 Hz at the left caudal vagus ends for 20 minutes before and after occlusion. (3) Sham control group (group SC): sham operation and sham stimulation. Carotid artery was cannulated for mean arterial pressure (MAP) monitoring. A strip of small intestine was taken from distal end of ileum for light microscopic (LM) and transient electron microscopic (TEM) examination at the time of 2 h after reperfusion. Improved Chiu’s scale was used to quantitatively assay the damage degree. The levels of malondialdehyde (MDA) and TNF-? in plasma were detected. RESULTS: MAP in every group kept steady during ischemia, but decreased gradually with the prolongation in the time of reperfusion. MAP decreased more dramaticly in group I/R than that in group VNS (P
