Effects of aplastic anemia patient serum on the expression of the crucial cyclin D isoform in cord blood CD34~+ cells
- VernacularTitle:再生障碍性贫血患者血清对脐血CD34~+细胞cyclin D关键亚型表达的影响
- Author:
Fankai MENG
;
Xiyou TAN
;
Hanying SUN
;
Wenli LIU
;
Jianfeng ZHOU
;
Dan LIU
;
Li FU
;
Lin LUO
;
Lan SUN
- Publication Type:Journal Article
- Keywords:
Anemia, aplastic;
Stem cells;
Cyclin D
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the pathogenesis of aplastic anemia (AA), we identified the crucial isoform of cyclin D that determine the proliferation of the cord blood CD34~+ cells and observed effects of AA serum on the expression of crucial cyclin D isoform in umbilical cord blood CD34~+ cells. METHODS: The CD34~+ cells were isolated with MIDI-MACS system. The isoforms of cyclin D were detected by RT-PCR and Western blotting. Methylcellulose culture system was used to measure the formation of CFU-GM. The expression level of crucial cyclin D isoform was assayed by RT-PCR and Western blotting after the CD34~+ cells were incubated in AA serum. RESULTS: The crucial cyclin D isoform in CD34~+ cells was cyclin D3. The AA serum inhibited the formation of CFU-GM and down-regulated expression level of the cyclin D3 from the mRNA to protein level, respectively. CONCLUSION: The AA serum inhibits the proliferation of CD34~+ cells and down-regulates level of cyclin D3, this may be one of hematopoiesis inhibition mechanisms in AA.
