Effects of high hydrostatic pressure on ADMA metabolism in human vascular endothelial cells and the role of RAS
- VernacularTitle:高静水压对血管内皮细胞ADMA水平的影响及RAS的作用
- Author:
Fang RAO
;
Hai SU
;
Juxiang LI
;
Ling WANG
;
Chunhua XIA
;
Xiaoshu CHENG
- Publication Type:Journal Article
- Keywords:
Pressure;
Endothelial cells;
Renin-angiotensin system;
Arginine
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of high hydrostatic pressure on asymmetric NG, NG-dimethyl-L-arginine (ADMA) metabolism of human vascular endothelial cells (HUVECs), and the role of renin-angiotensin system (RAS). METHODS: Cultured HUVECs of 3-6th passage were exposed to atmosphere (0 mmHg, APC), 120 mmHg (MPC), 180 mmHg (HPC). There were three groups in each pressure condition, one as control, the other two were interfered with captopril (Cap, 10 ?mol/L or 100 ?mol/L) or irbesartan (Irb, 10 ?mol/L or 100 ?mol/L) respectively. Cell proliferation was quantified by determining hexosaminidase activity at 12 h. Concentration of ADMA in conditioned medium was measured by high performance liquid chromatography (HPLC) at 12 h. RESULTS: Compared with APC group, ADMA concentration increased prominently in MPC and HPC (4.69?0.37 and 4.48?0.39 vs 0.75?0.05,P
