Role of neutrophil elastase and myeloperoxidase in asthma of rats
- VernacularTitle:中性粒细胞弹性蛋白酶、髓过氧化物酶在大鼠哮喘中的变化及意义
- Author:
Xiasheng TONG
;
Changchong LI
;
Zhengying RUAN
;
Hao CHEN
;
Haiyuan GUO
;
Guangmin FAN
- Publication Type:Journal Article
- Keywords:
Asthma;
Rats;
Neutrophils;
Leukocytes elastase;
Peroxidase
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the changes of polymorphonuclear leukocytes (PMN), neutrophil elastase (NE) and myeloperoxidase (MPO) expression in rat asthma. METHODS: Eighteen rats were randomly divided into two groups on average, including asthma group and control group. The rat model of asthma was established by the ovalbumin (OVA) challenge methods. Blood PMN were isolated and purified. The protein expression of MPO were detected by immunohistochemistry and chromatometry techniques. NE was detected by ELISA. RESULTS: (1) Immunohistochemistry showed that the levels of MPO expression around bronchus and in purified PMN in asthma group were significantly increased compared with those in control group (P
