The role of TGF-? signal protein Smad2/3 in tubulointerstitial fibrosis associated with unilateral ureteral obstruction in rats
- VernacularTitle:Smad2/3信号蛋白在大鼠梗阻性肾病肾组织中的表达
- Author:
Shenglang ZHU
;
Xueqing YU
;
Juping CHANG
;
Jiuhong LI
;
Jun ZHANG
;
Lu CHEN
- Publication Type:Journal Article
- Keywords:
Ureteral obstruction;
Transforming growth factor beta;
Smad2/3;
Kidney diseases
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the functional role of TGF-?_1 signal protein Smad2/3 in tubulointerstitial fibrosis associated with unilateral ureteral obstruction in rats. METHODS: The unilateral ureteral obstruction (UUO) model was induced by the ligation of left ureter. Rats were sacrificed at 1, 3, 7, 14, 21, and 28 days after UUO was initiated. TGF?_1 protein, phosphorylated Smad2/3 and interstitial ?-smooth muscle actin (?-SMA) expression were assayed by immunohistochemical staining. TGF-?_1 mRNA in the obstructed kidney was analyzed with in situ hybridration. HE and Masson staining were used for histological and morphometric studies of the pathological change in obstructed kidney. RESULTS: The results showed that upregulation of TGF-?_1 in tubulointerstitium of both cortex and medulla at day 3 (a 3.1 fold increase vs control, P
