A quantitative study of neuroglobin in cerebral cortex after traumatic brain injury in rats
- VernacularTitle:大鼠脑外伤后大脑皮质脑红蛋白的定量研究
- Author:
Xiangang DU
;
Peng GUAN
;
Xuemei CHEN
- Publication Type:Journal Article
- Keywords:
Forensic Pathology;
Traumatic brain injury;
Neuroglobin;
Immunohistochemistry;
Image analysis
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The amount of neuroglobin(Ngb) in cerebral cortex after different post-traumatic time intervals were studied in order to find out the value of Ngb in estimating the survival time after trauma.Methods Moderate brain contusions in rats were made using free-falling-body impact device,The area and gray values of Ngb immunoreactive products(Ngb-IR)in the core of injury in cerebral cortex and penumbra and the contra lateral cerebral cortex at different time points after trauma were studied by immunohistochemical method and image analysis.Results The expression of Ngb in the core of injured cerebral cortex declined quickly and reached the nadir by 24 hours and remained at low level for long time.On the contrary,it rose quickly and reached the peak value at 12 hours in the penumbra surrounding the core of injury,then declined gradually and reached normal level at 8d to 16d.Image analysis revealed that the expression of Ngb-IR in the penumbra of injured cortex were stronger than that in the opposite side.Conclusion The amount of Ngb in cerebral cortex at the core and penumbra of injury changed regularly according to different time intervals after trauma.Ngb may play an important role in neuroprotection during acute phase of injury.
