The Effect of Induced Differentiation of Abscisic Acid on Human HCC Cell Line SMMC-7721
- VernacularTitle:脱落酸对SMMC-7721人肝癌细胞诱导分化作用的研究
- Author:
Qiuye MA
;
Buqiang WU
;
Yonggang LU
;
Weiwei CHU
;
Yongzhang GUO
- Publication Type:Journal Article
- Keywords:
Abscisic acid;
Cell line SMMC-7221;
Induced differentiation
- From:
Journal of Kunming Medical University
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Study the effect of induced differentiation of abscisic acid (ABA) on human HCC cell line SMMC-7221.Methods Cultured SMMC-7221 cells were treated separately with RPMI-1640 culture medium, HMBA and ABA with different concentrations. Firstly, the appropriate concentration of ABA which inhibits SMMC-7221 cell proliferation was selected with the modified MTT method. Then electron microscopy was performed to observe the changes of microstructure. The cell cycle was analyzed by flow cytometry. Results Apart from 4?10 -4mmol/L concentration of ABA, the others could inhibit the cell proliferation. The inhibition rate increased with the time prolonging and the concentration increasing. The effect was most obvious with 4?10 -3mmol/L ABA. At this concentration the cells were arrested in G0/ G1 phase (P
