Feasibility of Detecting Bacterial Endotoxins in Low Molecular Weight Heparins Calcium Injection by TAL
- VernacularTitle:采用鲎试剂检查低分子量肝素钙注射液中细菌内毒素的可行性研究
- Author:
Guinan XIAO
;
Zhenlong LIU
;
Yankang XIE
- Publication Type:Journal Article
- Keywords:
Low molecular weight heparin calcium injection;
Bacterial endotoxin;
Kinetic turbidimetric assay;
Tachypleus amebocyte lysate
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the feasibility of detecting bacterial endoxins in low molecular weight heparins calcium injection LMWHC by tachypleus amebocyte lysate(TAL).METHODS:Bacterial endotoxins in samples were detected qualita?tively and quantitatively by kinetic turbidimetric assay and gel-clot method.RESULTS:Diluted to the concentration of25IU/ml,LMWHC had an obvious interference action to the TAL test,while not at the concentration of12.5IU/ml.The contents of bacterial endotoxins in all samples were less than0.01EU/IU.CONCLUSION:It is feasible to detect bacterial endotoxins in LMWHC by TAL test.
