Optimizing the Purification Technics of the Effective Blood-fat Dropping Parts from Radix Puerariae and Fructus Crataegi by Orthogonal Test
- VernacularTitle:正交试验优选葛根、山楂降脂有效部位的纯化工艺
- Author:
Yadong MIAO
;
Zhen OUYANG
;
Taotao JIANG
;
Bin YUAN
- Publication Type:Journal Article
- Keywords:
Radix Puerariae;
Fructus Crataegi;
Macroporous resin;
Orthogonal test;
Purification
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the feasibility of purifying the effective blood-fat dropping parts from radix puerariae and fructus crataegi and to optimize the technical conditions and parameters.METHODS:Absorption capacities of four different macroporous resins on pueraria total isoflavones,crataegi total flavonoids and crataegi total triterpenic acid were compared.With adsorption quantity as index,optimization of the technics of purifying the effective blood-fat dropping parts from radix puerariae and fructus crataegi with macroporous resins were performed.RESULTS:AB-8resin showed a best adsorbability on the effective blood-fat dropping parts from radix puerariae and fructus crataegi,its optimum purification condition was the following,the concentration of the stock solution was0.13g crude drug/ml with a flow rate at0.5ml/min,and its pH value at about3.5(the same as the stock solution);80%ethanol solution was taken as the eluting solvent at an eluting flow rate of2ml/min.The purity coefficient of the purified effective portions was above85%.CONCLUSIONS:AB-8macroporous resin could be used for the purification of the effective blood-fat dropping parts from radix puerariae and fructus crataegi,the process is feasible and the reproduction of resin is easy.
