Identification of proteins involved in insulin stimulation in vascular smooth muscle cells of SHR rats
- VernacularTitle:调控胰岛素致自发性高血压大鼠VSMC增殖的蛋白
- Author:
Xukai WANG
;
Yan WANG
;
Chengming YANG
;
Chunjiang FU
;
Ye ZHANG
;
Zhuoyu HE
- Publication Type:Journal Article
- Keywords:
Vascular smooth muscle cells;
Insulin;
Electrophoresis;
Phenotype
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To identify proteins involved in insulin stimulation and the molecular mechanism of proliferation and migration in vascular smooth muscle cells (VSMCs). METHODS: A series of methods, including 2-D electrophoresis, PDQuest software analysis of 2-DE gels, peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and SWISS-PROT database searching, were used to separate and identify the differentially expressed proteins. The difference of some proteins was proved by Western blotting. Proliferation and migration of VSMCs treated with insulin were also observed. RESULTS: DNA synthesis were increased in VSMCs. ~3H-thymidine incorporation in VSMCs from SHR (14.40?0.85) was higher than that in VSMCs from WKY (9.21?0.93, P
