Effect of bone marrow stromal cells on the anoxia cardiomyocytes in vitro
- VernacularTitle:骨髓间质细胞对体外培养的缺氧心肌细胞影响的初步研究
- Author:
Xuewen LIU
;
Xiangfeng CONG
;
Shengshou HU
;
Hao ZHANG
;
Xiaoling ZHANG
;
Xi CHEN
- Publication Type:Journal Article
- Keywords:
Cardiomyocytes;
Apoptosis;
Bone marrow stromal cells;
Anoxia;
Protein Bcl-2;
Protein Bax
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the influence of bone marrow stromal cells (BMSCs) on the anoxia cardiomyocyte apoptosis. METHODS: Using the anaerobic culture apparatus, the apoptosis of the cardiomyocytes, the BMSCs alone and co-cultured with each other were detected by morphological observation, PI staining flowcytometry, electrophoretic gel mobility analysis of DNA fragmentation. Western blotting was used to detect Bax and Bcl-2 protein expression. RESULTS: Compared with control, the BMSCs were unsensitive to anoxic cultured while the anoxic cardiomyocytes were prone to apoptosis. Apoptosis of cardiomyocytes was increased significantly, detected by PI staining and agarose gel elestrophoresis showed “DNA ladder”. However, when anoxia cardiomyocytes co-cultured with BMSCs, apoptosis cells were decreased, “DNA ladder” disappeared and the expression of protein Bax was also decreased. CONCLUSION: Bone marrow stromal cells prevent the anoxia cardiomyocytes from apoptosis, probably by suppressing the expression of Bax protein.
