Expression of glycine receptor in rat cardiomyocytes
- VernacularTitle:甘氨酸受体在大鼠心肌细胞的表达
- Author:
Junyan ZHANG
;
Daxiang LU
;
Renbin QI
;
Huadong WANG
;
Yanping WANG
;
Yongmei FU
;
Mengfei CHEN
;
Xue ZHAO
;
Chujie LI
- Publication Type:Journal Article
- Keywords:
Receptors, glycine;
Blotting, Western;
Rats;
Cardiomyocytes
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression of glycine receptor (GlyR) in adult rat cardiac tissue and cultured cardiomyocytes from neonatal rat hearts. METHODS: Total RNA and membrane protein were extracted from cardiac tissue of adult rats and primary cultured cardiomyocytes from neonatal rat hearts, ?1 and ? subunits mRNA and protein of GlyR were detected with the method of reverse transcription nest DNA polymerase chain reaction (RT-PCR) and Western blotting. RESULTS: The mRNA and protein expression of ? subunit were identified in the adult rat heart tissue and cultured cardiomyocytes, similar to the sequence and immunogenicity generated from GlyR ? subunit of the rat spinal cord; for ?1 subunit, mRNA was found in only cultured cardiomyocytes. CONCLUSION: These data demonstrate that adult rat cardiac tissue and cultured cardiomyocytes from neonatal rat hearts express mRNA and protein of GlyR subunits similar to the GlyR that expresses in the spinal cord, suggesting that GlyR exists in the membrane of the rat cardiomyocytes.
