Regulation of dopamine receptors on cocaine-induced MAPK signaling pathway and c-fos gene expression
- VernacularTitle:多巴胺受体在可卡因诱导的MAPK通路激活和c-fos基因表达中的作用
- Author:
Nuyun LIU
;
Lu ZHANG
;
Xiaoning WANG
;
Lin ZHANG
- Publication Type:Journal Article
- Keywords:
Cocaine;
Receptors, dopamine;
Genes, c-fos;
Gene expression;
Mitogen-activated protein kinases
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of D1 and D3 dopamine receptor on MAPK signal transduction and c-fos gene expression after acute cocaine treatment. METHODS: Activations of extracellular signal-regulated kinase (ERK), the c-Jun N-terminal kinase (JNK), p38 activation and expression of c-fos in wild type and D1 and D3 receptor mutant mice after acute cocaine treatment were checked by Western blotting. RESULTS: ERK activation and c-fos induction was enhanced in D3 mutant mice and abolished in D1 mutant mice by acute cocaine treatment, while p38 and JNK activation was not obviously modulated by the D1 and D3 receptors by acute cocaine treatment. Meanwhile, c-fos induction was inhibited when SL327, a specific MEK inhibitor, was injected before cocaine treatment. CONCLUSION: D1 and D3 receptors play opposite roles in the regulation of ERK activation and c-fos gene expression after acute cocaine treatment. The expression of c-fos gene depends on ERK signal pathway after acute cocaine treatment.
