Effects of acute HIV-1 infection on gene expression in U937 promonocytes
- VernacularTitle:人类免疫缺陷病毒-1型感染对U937前单核细胞基因表达的影响
- Author:
Shengting CHEN
;
Wangrong WEN
;
Yonghua ZHU
;
Juxiang LI
- Publication Type:Journal Article
- Keywords:
U937 cells;
HIV-1;
Gene expression;
Oligonucleotide array sequence analysis
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of acute HIV-1 infection on gene expression in U937 human promonocyte for understanding the pathogenecis of AIDS. METHODS: The expression levels of 550 host cell RNA transcripts in U937 human promonocyte at 2-3 d after HIV-1 infection were assessed using cDNA microarray analysis, and the results were confirmed by semiquantitative RT-PCR. RESULTS: Our results showed that 38 genes were differentially regulated in the infected U937 cells at 2-3 d post infection: 26 genes were down-regulated and 12 genes were up-regulated. These genes encode a host of proteins with divergent functions in a variety of cellular processes including receptor-mediated signaling transduction, subcellular signal trafficking, apoptosis, transcriptional regulation, and chemotaxis. CONCLUSION: HIV-1 infection alters gene expression in U937 human promonocytes.
