Effects of cotransplantation of rat bone marrow mesenchymal stem cells and bone marrow on hematopoiesis
- VernacularTitle:大鼠骨髓间质干细胞对同种骨髓移植后造血重建和免疫重建的作用
- Author:
Junxia LEI
;
Zhenyu GUO
;
Dongchang ZHAO
;
Hongxia LI
;
Weihua YU
;
Xiuming ZHANG
;
Qin ZHENG
;
Qing WEI
;
Shunong LI
;
Peng XIANG
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Bone marrow transplantation;
Hematopoietic remodeling;
Immune remodeling
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate effects of rBMMSC on hematopoiesis and immune reconstitution after allo-hematopoietic stem cells transplantation (HSCT). METHODS: Allogeneic BMT model from Fischer344 rats (RT-1Al) to Wistar rats (RT-1Au) was established. The effects of MSCs on hematopoietic reconstitution and immune reconstitution were studied by observing the survival rate, peripheral blood counts, thymus counts, spleen counts, bone marrow counts and immune function analysis at 30 days after transplantation. RESULTS: 1. Cotransplantation of MSCs and bone marrow (BM) was demonstrated to improve hematopoietic reconstitution. Lymphocyte and platelet counts in peripheral blood in cotransplantation groups were higher than those in control groups. More bone marrow neucleated cells were also observed in cotransplantation groups. 2. Cotransplantation of MSCs and BM improved immune reconstitution. First, overall thymic cellularity and spleen cellularity significantly increased in cotransplantation groups at day 30. Secondly, cotransplantation improved immune functional recovery. Non-specific lymphocytes proliferation reaction induced by ConA and LPS increased in cotransplantation group, and so did for allogeneic mixed-lymphocyte reaction. CONCLUSION: Hematopoietic reconstitution and immune reconstitution were significantly enhanced by MSCs cotransplanted with BM.
