Construction of the eukaryotic expression vector of mouse Sim2 and its effect on cell cycle in PC12 cells
- VernacularTitle:小鼠Sim2真核表达载体的构建及其对PC12细胞周期的影响
- Author:
Xianfang MENG
;
Jing SHI
;
Xiaochun LIU
;
Lingli LI
;
Bin PENG
- Publication Type:Journal Article
- Keywords:
Genes, Sim2;
PC12 cells;
Cell cycle;
Down syndrome
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of mouse Sim2 (mSim2) eukaryotic expression vector transfection on the cell cycle in PC12 cells in vitro and to explore the role of Sim2 in the pathogenesis of Down syndrome. METHODS: The full open reading frame of mSim2 was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into the vector pcDNA3. Then the constructed pcDNA3-Sim2 vectors were transiently transfected into PC12 with Lipofectamine~ TM . The expression of mSim2 was detected by RT-PCR. The effect of mSim2 on the cell cycle was observed by flow cytometry. RESULTS: The eukaryotic expression vector mSim2 was successfully constructed. There was notable expression of mSim2 in the cells transfected with pcDNA3-Sim2. There were more cells in G_0/G_1 phase in the pcDNA3-Sim2 transfected cells than that in the control (P
