Role of preconditioning to oxidative stress in HepG2 cells
- VernacularTitle:预适应对氧化应激中HepG2细胞保护作用的初步探讨
- Author:
Xuelin HAN
;
Xuemei CHEN
;
Fei ZOU
- Publication Type:Journal Article
- Keywords:
H_2O_2;
Stress;
Preconditioning;
Apoptosis;
HepG2 cells
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To induce preconditioning and oxidative stress by H_2O_2 in HepG2 cells. METHODS: The different doses of H_2O_2 were used to induce apoptosis in HepG2 cells, which was estimated by AO/EB staining, MTT assay and flow cytometry. RESULTS: The different group of HepG2 cells stained with AO/EB showed different staining state. The high dose of H_2O_2 resulted in the increase in apoptosis rate of HepG2 cells and made MTT activity decreased. However, after pretreated with low dose of H_2O_2, the apoptosis rate was decreased and MTT activity was increased. CONCLUSION: The high dose H_2O_2 induced apoptosis in HepG2 cells and the low dose H_2O_2 protected HepG2 cells against the oxidative stress.
