Effect of luteolin on metastasis of ovarian carcinoma cell line
- VernacularTitle:木犀草素对卵巢癌细胞株转移能力的影响
- Author:
Dakai XIAO
;
Yanmei QIN
;
Lier MO
;
Nianc LIANG
- Publication Type:Journal Article
- Keywords:
Luteolin;
Ovarian neoplasms;
Matrix metalloproteinases;
Neoplasms metastasis
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of luteolin on the metastasis of ovarian carcinoma HO-8910PM cells in vitro, and to elucidate its mechanisms. METHODS: The in vitro invasion and mobility of HO-8910PM were measured by Boyden chamber assay after exposure to luteolin for 6 h. Gelatinase activity was tested by gelatin zymography assay. The expression of tissue inhibitor of metalloproteinase-1 (TIMP-1), nm23 mRNA and that of ERK2 protein were tested by RT-PCR and Western blotting, respectively. RESULTS: Luteolin significantly inhibited in vitro invasiveness and mobility in HO-8910PM cells in a dose dependent manner as measured by Boyden chamber assay. Luteolin inhibited the MMP-9 secretion from HO-8910PM cells. Luteolin did not affect the mRNA expression of TIMP-1 and nm23. Luteolin also decreased ERK2 expression. CONCLUSION: Luteolin dose-dependently inhibited the metastasis of HO-8910PM cells in vitro. Inhibition of MMP-9 secretion and ERK2 expression may be involved in the anti-metastasic process of luteolin.
