Effect of sonic hedgehog signal pathway blocking on the proliferation and apoptosis of pancreatic cancer cell line SUIT-2
- VernacularTitle:特异性阻断Shh信号通路对胰腺癌细胞系SUIT-2增殖和凋亡的影响
- Author:
Weiguo HU
;
Chunyou WANG
;
Tao LIU
;
Gang ZHAO
- Publication Type:Journal Article
- Keywords:
Pancreatic Neoplasms;
Cyclopamine/ther use;
Signal Transduction;
Cell Division;
Flow Cytomtyry;
Models Animal
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of cyclopamine,the inhibitor of Sonic hedgehog signaling pathway,on the proliferation and apoptosis of pancreatic cancer cell line SUIT-2.Methods The SUIT-2 cells used in the experiment were cultured in vitro,and the MTT assay was used to examine the(antiproliferative) effect of cyclopamine.Flow cytometry(FCM) was used to examine the effects of cyclopamine on the proliferation index(PI) and apoptosis index(AI) of SUIT-2 cells.Nude mice tumor graft model was used to determine the effect of cyclopamine on growth inhibition of tumor graft.Results Cyclopamine(produced) time and concentration dependent antiproliferative effects on SUIT-2 cells.Cyclopamine could induce apoptosis and block cell cycle at G_0/G_1 phase.In cyclopamin treated SUIT-2 cells,the apoptosis index(AI) was 14.3?0.35,and proliferation index(PI) was 36.1?0.44;and in control group,the AI was 1.3?0.24,the PI was 52.3?0.28(all P
