Effect of gene transfer of antisense hypoxia inducible factor-1? on chemosensitivity of human pancreatic cancer cell line BxPC-3
- VernacularTitle:反义缺氧诱导因子-1?对胰腺癌细胞BxPC-3化疗敏感性的影响
- Author:
Qing CHANG
;
Renyi QIN
;
Jun GAO
;
Yanping FENG
;
Tao HUANG
- Publication Type:Journal Article
- Keywords:
Pancreatic Neoplasms/durg ther;
Antisense Hypoxia inducible factor-1?;
Chemosensitivity;
Gene therapy
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of antisense hypoxia inducible factor-1?(HIF-1?) on (chemosensitivity) of human pancreatic cancer cell line BxPC-3 under hypoxia. Methods BxPC-3 cells were divided into 3 groups:(1)BxPC-3 cells were non-transfected with antisense HIF-1? plasmid and exposed to 0.5% O_2 for 4hr(hypoxia control);(2)normoxic BxPC-3 cells were non-transfected with antisense(HIF-1?) plasmid(normoxia control);(3)BxPC-3 cells were transfected with antisense HIF-1? plasmid and exposed to 0.5% O_2 for 4hr(experimental group).Expression of HIF-1? and survivin was detected by RT-PCR and Western Blot.Growth inhibition rates and apoptosis rates of BxPC-3 cells under different(dosages) of chemotherapeutic agents(5-fluorouracil,doxorubicin and gemcitabine) were measured by MTT(colorimetric) assay and flow cytometry (FCM).Results Expression of HIF-1? was obviously down-regulated and at the same time susvivin expression was markedly down-regulated in experimental group(P
