Outcomes of preimplantation genetic diagnosis using either zona drilling with acidified Tyrode's solution or partial zona dissection.
10.5653/cerm.2012.39.3.118
- Author:
Hyun Jung KIM
1
;
Chung Hyon KIM
;
Soo Min LEE
;
Seung Ah CHOE
;
Joong Yeup LEE
;
Byung Chul JEE
;
Doyeong HWANG
;
Ki Chul KIM
Author Information
1. Institute of Fertility and Genetics, Hamchoon Women's Clinic, Seoul, Korea. kck57@hamchoon.com
- Publication Type:Original Article
- Keywords:
Embryo biopsy;
Partial zona dissection;
Zona drilling;
Acid Tyrode's solution
- MeSH:
Blastocyst;
Embryonic Structures;
Family Characteristics;
Herpes Zoster;
Isotonic Solutions;
Mandrillus;
Pregnancy;
Pregnancy Rate;
Preimplantation Diagnosis;
Prostaglandins D;
Zona Pellucida
- From:Clinical and Experimental Reproductive Medicine
2012;39(3):118-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To review the outcomes of preimplantation genetic diagnosis (PGD) using zona drilling with acid Tyrode's solution (chemical zona pellucida drilling, chemical ZD) and those of partial zona dissection (PZD). METHODS: Clinical outcomes of seventy-one couples undergoing 85 PGD cycles from January 2005 to December 2010 were included. Blastocyst formation and the hatching rate, clinical pregnancy rate, ongoing pregnancy rate, implantation rate, and fetal gender ratio of the PZD and chemical ZD groups were compared. RESULTS: Application of PZD resulted in a significantly higher rate of clinical pregnancy (40.7% vs. 15.4%, p=0.022), ongoing pregnancy (35.6% vs. 11.5%, p=0.023), and implantation (18.1% vs. 5.7%, p=0.007) compared with chemical ZD. Among non-transferred embryos, the rate of blastocyst formation on day 5 (49.1% vs. 39.5%, p=0.016) and hatching on day 6 (47.2% vs. 26.5%, p<0.001) were also significantly higher in the PZD group. CONCLUSION: The mechanical zona dissection method showed better outcomes than chemical ZD in terms of the blastocyst development and pregnancy rate. In this study, the fact that chemical ZD was conducted in different period from mechanical method should be considered in interpreting the result.
