Effect of perforin and granzyme B in peripheral blood lymphocytes during acute rejection in renal transplantation
- VernacularTitle:外周血淋巴细胞穿孔素和颗粒酶B检测在诊断移植肾急性排斥反应中的价值
- Author:
Jiangping CHANG
;
Feng WANG
;
Ming SHI
;
Xintao ZHANG
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
Graft rejection;
Membrane glycoproteins;
Serine endopeptidases;
Lymphocytes
- From:
Journal of Chinese Physician
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of perforin and granzyme B in peripheral blood lymphocytes during acute rejection in renal transplantation.Methods Sixty-seven recipients of renal allograft were involved in the study.The recipients were divided into four groups: group 1 with 7 cases of acute rejection,group 2 with 8 cases of delayed graft function,group 3 with 27 cases of stable function and group 4 wih 25 cases of long-term survival.The expressions of perforin and granzyme B of peripheral blood lymphocytes were studied by quantitative reverse transcription polymerase chain reaction(RT-PCR).Results The expressions of perforin and granzyme B in acute rejection group were significantly higher than those of the other three groups(P
