A study on the influential factors of G6PD/6PGD specific value assay in the heterozygotes of G6PD gene variants in female patients
- VernacularTitle:G6PD比值法检测葡萄糖-6-磷酸脱氢酶基因突变女性杂合子影响因素的研究
- Author:
Yunsheng CHEN
;
Weiying JIANG
;
Changgang LI
- Publication Type:Journal Article
- Keywords:
Glucosephosphate dehydrogenase;
Glucosephosphate dehydrogenase deficiency;
Genes;
Mutation;
Heterozygote
- From:
International Journal of Laboratory Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the detection of G6PD heterozygotes in female patients by the optimum experimental factors of G6PD/6PGD specific value assay.Methods (1)Identifying the mutations of G6PD gene with the use of amplified refractory mutation system (ARMS).(2)Measuring the G6PD/6PGD specific value.Results According to the data analysed by statistics and ROC curve, the optimum experimental factors included that the incubation temperature was 37℃,the substrate concentrations were 0.78 mmol/L G6PNa2 and 0.195mmol/L NADP+, the reaction time was 10min.Conclusion The optimum experimental factors of G6PD/6PGD specific value assay may be used to improve the detection rate of G6PD heterozygotes in female patients.
