A preliminary study on factors influencing the outcomes of activities of CK-isozyme
- VernacularTitle:影响肌酸激酶同工酶活性检测结果的因素探讨
- Author:
Wanjun ZHENG
;
Minru DENG
;
Li WONG
- Publication Type:Journal Article
- Keywords:
Creatine kinase;
Immunoinhibition
- From:
International Journal of Laboratory Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the false positive of enzyme linked immunoinhibition continuous assays to detect CK-MB in clinic,to reasonably explain the results of detection and to get correctly the project of solving.Methods A total of 125 serum specimen of non-myocardial infarction patients were detected by enzyme linked immunoinhibition continuous assays,in retrospective study.To analyze the factors which made the CK-MB enzymatic activity unit pseudo-rising.Results Total CK-MB enzymatic activity was low in 86 serum specimen of adult patients with non-myocardial infarction,but in the patients with tumor,encephalic infarction and allergic disorder the pseudo-positive ratio of the CK-MB enzymatic activity was high.There were 53 cases, in which CK-MB activity accounted for 6%-21% enzymatic activity of CK, and 6 cases accounted for more than 38% in 59 cases in which the CK-MB activity was more than 5% enzymatic activity of CK.In 38 neonate cases, the enzymatic activity of CK ranged from 145U/L to 1974U/L,In 1 case of neonatal respiratory distress syndrome CK-MB activity accounted for more than 90% enzymatic activity of CK.Another haemolytic sample led to false positive result.Conclusion The phenomenon of enzymatic activity rising in the serum specimen of non-myocardial infarction patients may be caused by the factors of lots CK, CK-BB and AK in serum.Those materials maybe have effects on the action between McAb and M subunit of the CK.Some non-inhibit CK-M and the M subunit with B subunit may participate the enzymatic activity together.Because of the shortcoming of method,the single assay should not be taken as the diagnostic basis.
