Preparation of Oral Vaccine Microspheres for Newcastle Disease Virus
- VernacularTitle:鸡新城疫病毒口服微球的制备研究
- Author:
Qiaohong HU
- Publication Type:Journal Article
- Keywords:
Newcastle disease virus;
Oral vaccine microspheres;
Hemaggltination;
Valence
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the preparation technique for oral vaccine microspheres.METHODS:Newcastle dis-ease virus(NDV)vaccine was used as model drug,and PLGA as carrying agent to prepare NDV-PLGA oral microspheres by W/O/W emulsifying-solvent evaporation technique.Orthogonal design experiment was carried out to determine the influence of NDV volume,PLGA concentration,stirring speed and protectants at inner aqueous phase on the shape,size,adhesion and activity of microspheres.The valence of drug release in microspheres was measured by hemagglutination.RESULTS:The best condition for preparing NDV microspheres was found to be200?l of NDV,4%PLGA,stirring speed at8000r/min,and BSA as protectant.CONCLUSION:The established technique is the optimal choice for preparation of oral vaccine micro-spheres.
