The inhibition of laryngeal squamaous cell carcinoma by endostatin
- VernacularTitle:内皮抑素体外抑制喉鳞状细胞癌细胞的效果观察
- Author:
Dejun JIN
;
Yu WANG
;
Hui XIAO
;
Chao WANG
;
Jingjing HE
;
Xinghan LIU
- Publication Type:Journal Article
- Keywords:
Endostatins;
Laryngeal Neoplasms;
Carcinoma,Squamous Cell;
Umbilical Veins;
Endothelial Cells;
Apoptosis
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the inhibitory effect and possibility inhibitory mechanisms ofendostatin on laryngeal carcinoma cells (Hep-2) and human umbilical vein endothelial cells (HUVEC). METHODS ①Using the MTT assay , the effect of different concentrations of endostatin (10 ?g/ml～50 ?g/ml) and endostatin (30 ?g/ml) with different contact times (24～72h) on the livability of Hep-2 and HUVEC cells were determined; ②Changes in the ultrastructures of Hep-2 and HUVEC cells were examined by electron microscopy; ③Survivin mRNA content was determined in Hep-2 cells exposed to endostatin (30 ?g/ml) by RT-PCR test; ④The effect of endostatin (30 ?g/ml) on the ectogenetic artificial blood vessel models was observed by light microscopy. RESULTS ① The growth of Hep-2 and HUVEC cells was significantly inhibited (P
