SEM observation of hair cell progenitors forced to express Hath1 from rat cochleae
- VernacularTitle:Hath1基因转染后大鼠耳蜗毛细胞前体细胞的扫描电镜观察
- Author:
Yuan ZHANG
;
Jianhe SUN
;
Wei GUO
;
Yinyan HU
;
Suoqiang ZHAI
- Publication Type:Journal Article
- Keywords:
Hair Cells;
Regeneration;
Microscopy,Electron,Scanning;
Cilia
- From:Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of Hath1 on epithelial ridge (GER) cells from postnatal rat cochlear. METHODS An experimental method was developed which allowed the isolation and culture of GER cells from P1 rat cochleae using a combinatorial approach of enzymatic digestion and mechanical separation. The dissociated GER cells were cultured in DMEM+10% FBS. The GER explants infected with ad-Hath1 for 3 hours were cultured for 10 days and observed under scanning electron microscope. RESULTS The GER cell cultures tended to attach to the substratum and grow in patches which assume a polygonal morphology similar to that of epithelial cells in medium containing 10% serum. Stereociliary bundle-like structures were observed in the boundary of GER cell patches forced to express Hath1. CONCLUSION That GER cells which are likely hair cell progenitors could be cultured in vitro and generate stereociliary bundle-like structure when forced to express Hath1 suggests that the misexpression of Hath1 probably can induce the predifferentiation of pure hair cell progenitors into hair cells in vitro.
