Study on apoptosis of K562 cells induced by tumor-bearing herb serum of selenium-protein polysaccharide extracted from Qinba selenium-mushroom(秦巴硒菇)
- VernacularTitle:秦巴硒菇提取物硒蛋白多糖荷瘤药物血清促慢性红白血病细胞株K562凋亡的实验研究
- Author:
Huiyuan CHU
;
Che CHEN
- Publication Type:Journal Article
- Keywords:
Qinba selenium-mushroom;
selenium-protein polysaccharide;
apoptosis;
caspase3
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of selenium-protein polysaccharide extracted from Qinba (selenium-mushroom)(秦巴硒菇) on apoptosis of K562 cells.Methods: Tumor-bearing mice model was established,and selenium-protein polysaccharide extracted from Qinba selenium-mushroom contained-herb serum was prepared by the method of serum pharmacology.K562 cells were conventionally cultured.Growth inhibition rate of tumor cells was detected by methyl thiazolyl tetrazolium(MTT) method.Morphological changes of apoptotic cell were observed under a fluorescent microscope.Cell apoptosis was observed by DNA electrophoresis.Caspase3 relative gene was measured by colorimetry.Results: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could significantly inhibit the growth of K562 cells,there was a remarkably positive correlation between drug concentration,time and inhibitory rate.Apoptotic phenomenon was certained via morphological examination and DNA electrophoresis.Compared with control group,caspase3 gene was markedly upregulated.Conclusion: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could induce apoptosis of K562 cells.The mechanism may be related to upregulation of caspase3.
