The establishment of multidrug resistant model in nude mice via orthotopic unplantation of human multidrug resistant hepatocellular carcinoma cells
- VernacularTitle:裸鼠原位肝细胞癌多药耐药模型的建立
- Author:
Lei DING
;
Xiaoping CHEN
;
Zhiwei ZHANG
;
Wanguang ZHANG
;
Zhihui WANG
;
Chunlei LI
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Drug resistance, multiple;
Model, animal;
Nude mice
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish multidrug resistant (mdr) model in nude mice via orthotopic implantation of human multidrug resistant hepatocellular carcinoma cells. Methods Orthotopic mdr1 hepatoma were obtained by injecting human hepatocellular carcinoma cells HepG2 and multidrug resistant human hepatocellular carcinoma cells HepG2/ADM subserosally into the mouse liver. Ultrasonography and laparotomy were used to detect tumor growth, and integrity of MDR1 gene from multidrug resistant human hepatocellular carcinoma cells HepG2/ADM. Implantated tumor tissues were amplificated by long PCR technique and sequenced. Furthermore, the MDR1 mRNA and P-gp protein expression were evaluated by reverse transcription and polymerase chain reaction ( RT-PCR )、 Western blotting、immunohistochemistry ( IHC). Results The successful rate of tumor implantation was 100% and multidrug resistant induced rate was 95% (19/20) respectively. 3. 8 kb mdrl integrity gene band was identified from both HepG2/ADM cells line and corresponding implantated tumor tissues by long RT-PCR technique. MDR1 gene sequence was in coincidence to that reported in genebank. The expression of MDR1 mRNA and P-gp protein in multidrug resistant groups were significantly higher than that in control groups. P-gp protein expression in multidrug resistant groups (42.6% ? 1.7%) was higher than controls (2.6% ? 0. 1%), P
