PGE_2 promotes the migratory ability and immunological effect of bone marrow-derived dendritic cells loaded with breast carcinoma antigen
- VernacularTitle:前列腺素E_2对小鼠树突状细胞迁移能力及抗乳腺癌免疫作用的影响
- Author:
Xun ZHU
;
Linlin ZHEN
;
Wei ZHENG
;
Hanjing WANG
;
Xuanyi WANG
;
Zhengyan WU
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Receptor, chemokines;
Immunological therapy;
Dendritic cells
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To promote the migratory ability and immunological effect of bone marrow-derived dendritic cells ( BMDC) loaded with breast carcinoma antigen. Methods DCs were cultured by the medium containing rmGM-CSF and rmIL-4. After loaded with breast carcinoma antigen, DCs were stimulated with PGE2 for 1day. CD86, CD80, and CCR7 were measured by flow cytometry. The expression of CCR7 on surface of BMDC was also detected by RT-PCR and Western blotting. The chemotaxis assay was measured by migration through a polycarbonate filter in transwell chambers. The competence of inducing mixed lymphocyte response (MLR) and specific cytotoxic T lymphocyte ( CTL) were detected with MTT. The effect of DC blocking tumor growth in breast carcinoma model were also studied. Results Compared with control group, PGE2 upregulated surface markers of CD86, CD80, and CCR7 (P
