Protective effects of etomldate on the cortex and hippocampus against anoxia-reoxygenation injury in rats
- VernacularTitle:依托咪酯对大鼠皮层、海马脑片缺氧复氧损伤的保护作用
- Author:
Yongqian SHEN
;
Qingsheng XUE
;
Hongzhuan CHEN
- Publication Type:Journal Article
- Keywords:
Etomidate;
Cerebral cortex;
Hippocampus;
Hypoxia,brain;
Reperfusion injury
- From:
Chinese Journal of Anesthesiology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of etomidate on the cortex and hippocampus against anoxia-reoxygenation (A/R) injury.Methods Male adult SD rats weighing 90-100 g were anesthetized with ether and decapitated. Their brains were immediately removed. Cortical and hippocampal slices were prepared and were randomly divided into 6 groups: group Ⅰ control; groupⅡ A/R; in group Ⅲ - Ⅵ the brain slices were first incubated in the presence of etomidate 3, 6, 15 ?mol?L-1 or etomidate 6 ?mol?L-1 + picrotoxin 50 ?mol?L-1 (GABA receptor antogonist) for 30 min. Then the slices were subjected to 10 min anoxia (95% N2 +5% CO2) followed by 120 min reoxygenation. The absorbance value (A490) of TTC staining (2. 3. 5-triphenyl tetrazolum chloride) and intracellular free Ca2+ ([Ca2+]i) accumulation were determined. Results The A490 in cortical and hippocampal slices were significantly decreased while [Ca2+] i significantly increased in A/R group as compared with control group. Different concentrations of etonlidate attenuated the changes induced by A/R especially 6 ?mol?L-1. The protective effects of etomidate could be antagonized by GABAA antagonist. Conclusion Etomidate can protect the cortex and hippocampus against A/R injury to some extent by acting on GABAA recoptor and decreasing intracellular Ca2+ overloading.
