Effects of heterogously-expressed RON receptor tyrosine kinase on motile/invasive ability of human colorectal cancer cell line RKO
- VernacularTitle:RON受体酪氨酸激酶的过表达对结肠癌细胞移动/浸润能力的作用
- Author:
Weidong DU
- Publication Type:Journal Article
- Keywords:
Protein-tyrosine kinase;
siRNA;
Cadherins;
Colorectal neoplasms;
Neoplasm invasiveness;
Cell movement
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the roles of overexpression of RON receptor tyrosine kinase in motile/invasive ability of human colorectal cancer cell line RKO.METHODS: A eucaryotic expression vector pDR2 containing full-length wt-RON cDNA was transfected into the colorectal cancer cell line RKO and a stable expression clone was obtained.The motile/invasive ability was tested by wound healing test and the transwell migration assay.The expression of E-cadherin was measured by Western blotting.RESULTS: Motile ability of transfected RKO was greatly promoted by transwell chemotaxis assay(P
