Changes of mitochondrial membrane potential and mitochondrial mass in camptothecin-induced Jurkat cells
- VernacularTitle:喜树碱诱导Jurkat细胞线粒体膜电势和线粒体质量变化的研究
- Author:
Yingjuan JIANG
;
Yaoying ZENG
;
Tong WANG
;
Jingxian ZHAO
;
Feiyue XING
;
Xichao WANG
;
Peiyan LIANG
- Publication Type:Journal Article
- Keywords:
Camptothecin;
Jurkat cells;
Mitochondrial;
Membrane potentials;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the changes of mitochondrial membrane potential(△?m) and mitochondrial mass in apoptosis of Jurkat cells induced by camptothecin(CPT).METHODS: Jurkat cells were treated with CPT.Annexin V-FITC/propidium iodine(PI) double stainig was used to detected early stage of apoptosis and PI staining for analyzing the cell cycle.Jurkat cells were stained by annexin V-PE/DiOC_6(3) to detect changes of △?m.The mitochondrial mass was measured by cytometry with NAO staining.RESULTS: 6 h after treated with 10 ?mol/L CPT,the rate of early apoptotic cells(22.59?1.04)% had significantly difference compared with control group(3.93?0.73)%(P0.05).Apoptotic peak appeared obviously after treated with CPT,the percentage of late apoptotic cells(13.58?0.97)% had distinctly difference compared with control group(3.18?0.51)%(P
