Construction and identification of mouse telomerase reverse transcriptase eukaryotic expression vector
- VernacularTitle:小鼠端粒酶蛋白亚单位真核表达载体的构建和序列测定
- Author:
Nan JIANG
;
Guihua CHEN
;
Minqiang LU
;
Yang YANG
;
Changjie CAI
;
Hua LI
;
Chi XU
;
Shuhong YI
;
Genshu WANG
;
Huimin YI
;
Lianqiang FENG
- Publication Type:Journal Article
- Keywords:
Telomerase;
Oligonucleotide array sequence analysis;
Eukaryotic expression vector
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To clone and express mouse telomerase reverse transcriptase(mTERT) cDNA and obtain eukaryotic expression vector.METHODS: The cDNA of mTERT was amplified by RT-PCR and PCR.After purification,the gene fragment was cloned into a vector PUC-19.The sequence of inserted mTERT gene fragment was also detected.RESULTS: The recombinant plasmid PUC-19-mTERT was constructed.Positive clones were screened and identified by PCR and digestion with restriction enzyme.The size of gene fragment was(3 369) bp and in accordance with the expected one.CONCLUSION: The mTERT cDNA was obtained and the recombinant eukaryotic expression vector was successfully constructed.The study lays foundation for DCs vaccine modified by mTERT gene for the treatment of tumor.
