Differentiation of CD34~+ cells in human umbilical blood to eosinophils under the condition of cell culture in vitro
- VernacularTitle:人脐带血CD34~+细胞体外诱导分化为嗜酸粒细胞的研究
- Author:
Hui ZHANG
;
Daliang ZHANG
;
Chenghao GUO
- Publication Type:Journal Article
- Keywords:
CD34~+ cells;
Eosinophils;
Fetal blood
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate differentiation of CD34~+ cells in human umbilical blood into eosinophils under the condition of cell culture in vitro.METHODS: CD34~+ cells were separated and purified from human umbilical blood.The cells were divided into negative group,IL-5 group and allergic rhinitis serum group.The differentiation ability of the cells was measured by flow cytometry,HE staining and electron microscope at the first day,second day, 7th day,14th day and 28th day culture.RESULTS: The proportion of CD34~+ cells in IL-5 group and allergic rhinitis serum group were decreased at the second day.The proportion in allergic rhinitis serum group was lower than that in IL-5 group significantly.The typical structure of eosinophils was observed at the second day.CONCLUSION: The allergic patient serum and IL-5 induce differentiation of CD34~+ cells in human umbilical blood to eosinophils.
