PPARs ligands antagonizes Ang-induced decrease in endothelial NO production by upregulating eNOS expression
- VernacularTitle:PPARs激动剂上调内皮细胞表达eNOS并增加NO生成
- Author:
Shiming LIU
;
Yuexia DING
;
Yun ZHONG
;
Qicai LIU
;
Bing LI
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator-activated receptors;
Ciglitazone;
Fenofibrate;
Nitric-oxide synthase;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: We hypothesized that PPAR? ligands stimulate endothelial-derived nitric oxide(NO) release to protect the vascular wall.Thus,the purpose of this study is to investigate the effects of ciglitazone(Cig) and fenofibrate(Fen) on angiotensin Ⅱ(AngⅡ)-induced decrease in endothelial NO synthase(eNOS) expression and NO production in human umbilical vein endothelial cells(HUVECs).METHODS: HUVECs were preincubated for 24 h with Cig(10~(-7), 10~(-6),10~(-5),10~(-4) mol/L) or Fen(10~(-5) and 10~(-4) mol/L),then incubated for 12 h with 10~(-7) mol/L AngⅡ.Total RNA was extracted,and the expression of mRNA and protein of eNOS was assessed by RT-PCR and Western blotting.NO production was measured by Griees method.RESULTS: In the presence of 10~(-7) mol/L AngⅡ for 12 h,NO production in cultured HUVECs was decreased(P
