Effects of TGF-?_1 and signal protein Smad3 on rat cardiac myocyte hypertrophy
- VernacularTitle:TGF-?_1及其信号蛋白Smad3对大鼠心肌细胞肥大的作用
- Author:
Jun HUANG
;
Fangzhou CHENG
;
Junming LI
;
Yexin MA
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta;
Protein Smad3;
Myocardium;
Hypertrophy
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of TGF-?_1 and signal protein Smad3 on rat cardiac myocyte hypertrophy.METHODS: The total protein was analyzed by flow cytometry and the ANF mRNA expression was measured by RT-PCR to judge the hypertrophy of cultured neonatal cardiac myocytes.Smad3 mRNA expression in cardiac myocytes was measured by RT-PCR,and the protein expression of Smad3 was analyzed by Western blotting.RESULTS: TGF-?_1 significantly increased the total protein in cardiac myocytes and promoted ANF mRNA expression,compared with control group.In cultured neonatal myocytes,AS-ODN of Smad3 inhibited myocyte hypertrophy induced by TGF-?_1.Smad3 mRNA and protein expression increased at 15 min after incubated with TGF-?_1,reached the peak at 1 h,and declined at 4 h.CONCLUSION: TGF-?_1 and signal protein Smad3 may participate in the progress of rat cardiac myocyte hypertrophy.
