The protective effect of composite salviae dropping pills on human umbilical vein endothelial cells injured by lipopolysaccharide
- VernacularTitle:复方丹参滴丸对人血管内皮细胞功能及形态保护作用的研究
- Author:
Dongxia WANG
;
Xiaoming WANG
;
Jinglan XU
- Publication Type:Journal Article
- Keywords:
Salviae miltiorrhizae;
Umbilical vein endothelial cells;
Lipopolysaccharides;
Nitric oxide;
Endothelin-1
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the function and morphological change of human umbilical vein endothelial cells(HUVECs) treated with lipopolysaccharide(LPS) and composite salviae dropping pills(DSP).METHODS: HUVECs were cultured and incubated within 10 mg/L LPS for 12 hours.Different final concentrations of composite salviae dropping pills(1 g/L,0.5 g/L,0.25 g/L,0.1 g/L) were added before and after LPS treatment. Cell viability,NO,NOS,ET-1 and intracellular calcium were measured.The cells were observed under inverted microscope,inverted phase contrast microscope,laser confocal scanning microscopy and transmission electron microscope.RESULTS: When given after LPS treatment,different final concentrations of composite salviae dropping pills played a protective role(P0.05).The HUVECs injured by LPS underwent apparent morphological change after treatment with composite salviae dropping pills.CONCLUSION: Composite salviae dropping pills have an evident protective effect on human umbilical vein endothelial cells exposed to LPS.
