Gelatinases expression and their activity in rat alveolar macrophages induced by cigarette smoke medium
- VernacularTitle:香烟提出物对被动吸烟大鼠肺泡巨噬细胞源性明胶酶的表达和酶活性的影响
- Author:
Yaqing LI
;
Zhenxiang ZHANG
;
Yongjian XU
;
Shixin CHEN
;
Wang NI
;
Zhao YANG
;
Dan MA
- Publication Type:Journal Article
- Keywords:
Lung diseases,obstructive;
Macrophages,alveolar;
Gelatinases;
Smog
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of cigarette smoke medium(CSM) on the gene expression and activity of gelatinases from alveolar macrophages(AMs) in the rat,and then to explore their role in the pathogenesis of chronic obstructive pulmonary disease(COPD).METHODS: AMs were obtained from BALF of the rats that had smoked for 12 weeks.CSM was produced following the method of Wirtz and colleagues,and the cultured AMs were respectively stimulated for 24 h by 0%,1%,3%,5%,10%, 15% CSM.The mRNA levels of MMP-9 and MMP-2 were detected by semi-quantitative RT-PCR,and the enzyme activity was measured by Zymography.RESULTS: When the concentration of CSM was below 5%,the expression and activity of MMP-9 and MMP-2 signficantly increased with the concentration of CSM in a dose-depended manner(P
