Role of GATA-3 in the pathogenesis of airway inflammation in a rat asthma model
- VernacularTitle:GATA-3在大鼠哮喘模型气道炎症中作用的实验研究
- Author:
Qunyi DENG
;
Zhenxiang ZHANG
;
Yongjian XU
- Publication Type:Journal Article
- Keywords:
Asthma;
GATA-3;
Airway;
Inflammation;
Gene therapy
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of GATA-3 in the pathogenesis of airway inflammation in a Wistar rat asthma model.METHODS: The Wistar rat asthma model was made with conventional method and animals were divided into five groups(10 rats in each group): asthma group(A group),dexamethasone group(D group),antisense oligonucleotide group(AS group),nonsense oligonucleotide group(NS group) and normal control group(N group).Antisense,nonsense oligonucleotide were administered intranasally,and the dexamethasone was injected intraperitoneally.The airway inflammation was observed with HE staining method.The GATA-3 positive cells were stained immunohistochemically.The GATA-3 mRNA expression in pulmonary tissue was investigated with RT-PCR.The GATA-3 protein in pulmonary tissue was detected by Western blotting.RESULTS: In contrast to N group,the expression of GATA-3 mRNA, protein and the amount of inflammatory cells in pulmonary tissue in group A were increased significantly(P
