Hepatocyte growth factor protects hepatocytes from apoptosis induced by actinomycin D
- VernacularTitle:肝细胞生长因子拮抗放线菌素D诱导的肝细胞凋亡及机制探讨
- Author:
Wenjun LI
;
Xiaokun LI
- Publication Type:Journal Article
- Keywords:
Hepatocytes;
Apoptosis;
Signal transduction;
Hepatocyte growth factor;
Atinomycin D
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the anti-apoptotic effect of hepatocyte growth factor(HGF) on actinomycin D(ActD)-induced apoptosis in hepatocytes and the possible pathway.METHODS: Hepatocytes were exposed to ActD and HGF.The cytotoxic effects of ActD were tested by MTT.Apoptotic cells were identified by Hoechst 33342 staining,flow cytometry and detection of DNA fragmentation with agarose gel.Akt and phospho-Akt were detected by Western blotting analysis.RESULTS: The results showed that ActD induced apoptosis in hepatocytes 5 h after treatment.Phosphatidylinositol-3 kinase(PI-3K) specific inhibitor wortmannin enhanced the apoptotic effect of ActD.Furthermore, HGF significantly reduced the apoptosis in hepatocytes induced by ActD in a concentration-dependent manner.In the presence of wortmannin,HGF did not overcome apoptosis.CONCLUSION: Wortmannin enhances the apoptotic effect of ActD.HGF protects hepatocytes from apoptosis induced by ActD through a PI3K/Akt pathway.
