Effect of diterpenoid compound 5F of Peris semipinnata L on apoptosis of human pterygium body fibroblasts in vitro
- VernacularTitle:半边旗提取物5F诱导人翼状胬肉成纤维细胞凋亡的研究
- Author:
Yudong CHEN
;
Yingfen WANG
;
Jiansu CHEN
;
Zhijie LI
;
Xiaolin CHEN
- Publication Type:Journal Article
- Keywords:
Pterygium;
Fibroblasts;
Apoptosis;
Drugs,Chinese herbal
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of diterpenoid compound 5F of Pteris semipinnate L on apoptosis in cultured human pterygium body fibroblasts(HPFs).METHODS: Fibroblasts collected from human pterygium body were cultured in vitro with 5F at different time point.Transmission electron microscope(TEM) was used to observe the ultrastructure changes of the HPFs.The changes of the cell cycle and apoptosis of HPFs were evaluated with flow cytometry(FCM).RESULTS: 5F at the concentration of 32 mg/L induced HPF apoptosis at various time points.CONCLUSION: 5F induces HPFs apoptosis.Apoptotic cells are evolved to necrosis with the prolongation of the time.These findings indicate that 5F has a potential clinical value.
