Content Determination of Glycyrrhizic Acid in Glycyrrhizin Liposome by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定甘草酸脂质体中甘草酸的含量
- Author:
Benquan HU
;
Jianfeng CHENG
;
Mei LIU
;
Jianrong HE
;
Bishi WANG
;
Sheng YANG
- Publication Type:Journal Article
- Keywords:
Glycyrrhizic acid;
Liposome;
RP-HPLC;
Contents;
Entrapment rate
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an RP-HPLC method for the content determination of glycyrrhizic acid in gly-cyrrhizin liposome.METHODS:The separation was performed on Shim-pack VP-ODS column with methanol-buffer phosphate(pH=2.6,68∶32)as the mobile phase,the detection wavelength was254nm and the flow rate was1ml/min.RE-SULTS:Glycyrrhizic acid was well-separated with peaks of the adjuvants and the solvent.The linear range for glycyrrhizic acid was1～100?g/ml(r=0.9999,n=5).The average recovery was97.56%(RSD=1.03%).CONCLUSION:This method is accurate and sensitive,and suitable for the content determination of glycyrrhizic acid in glycyrrhizin liposome.
